Composite
Part:BBa_K5311017:Design
Designed by: Carlos Perez de Arenaza Group: iGEM24_UPF-Barcelona (2024-09-27)
MG_026 + RNA thermometer ForU + sfGFP + His-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 285
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For this composite part we have codon optimized the CDS for the RNA Thermometer to be more compatible with C. acnes, we also introduced the standard prefix and sufixes in our sequence to ensure a proper alignment for the Gibson Assembly.
Source
This part was created by using the corresponding sequences on this registry: MG26:BBa_K5311000 Part:; RNA Thermometer: Part:BBa_K115002; sfGFP: Part:BBa_K5311007 and the His-tag corresponds to a sequence of 6 Hystidines